| Date: | 12–17 May |
| Edition: | 18th |
| Draw: | 32S / 16D |
| Surface: | Clay |
| Prize Money: | $50,000 |
| Location: | Samarkand, Uzbekistan |
| Champs: |  Farrukh Dustov |
| Champd: |  Sergey Betov / Aliaksandr Bury |
The 2014 Samarkand Challenger was a professional tennis tournament played on clay courts. It was the 18th edition of the tournament which was part of the 2014 ATP Challenger Tour. It took place in Samarkand, Uzbekistan between 12 and 17 May 2014.
| Country | Player | Rank1 | Seed | |
|---|---|---|---|---|
| Uzbekistan | Denis Istomin | 55 | 1 | |
 Bosnia and Herzegovina | Damir Džumhur | 131 | 2 | |
 Austria | Gerald Melzer | 151 | 3 | |
| Uzbekistan | Farrukh Dustov | 165 | 4 | |
| Toni Androić | 222 | 5 | ||
 Canada | Filip Peliwo | 223 | 6 | |
| Hans Podlipnik Castillo | 224 | 7 | ||
 Spain | ||||
 Lithuania | Laurynas Grigelis | 232 | 9 |
The following players received wildcards into the singles main draw:
The following players received entry from the qualifying draw:
Anton Zaitcev Sanjar Fayziev
Denys Molchanov Alexander Lobkov| Country | Player | Country | Player | Rank1 | Seed | |
|---|---|---|---|---|---|---|
 Ireland | James Cluskey |  India | Saketh Myneni | 345 | 1 | |
| Belarus | Sergey Betov | Belarus | Aliaksandr Bury | 373 | 2 | |
| Uzbekistan | Farrukh Dustov | Ukraine | Denys Molchanov | 404 | 3 | |
| Antal van der Duim | Boy Westerhof | 417 | 4 |
The following pairs received wildcards into the doubles main draw:
Sarvar Ikramov / Djurabeck Karimov Shonigmatjon Shofayziyev / Vaja Uzakov Sanjar Fayziev / Temur Ismailov Farrukh Dustov def. Aslan Karatsev, 7–6(7–4), 6–1 Sergey Betov / Aliaksandr Bury def. Shonigmatjon Shofayziyev / Vaja Uzakov, 6–4, 6–3