| Date: | 15–21 September |
| Edition: | 20th |
| Surface: | Clay |
| Location: | Quito, Ecuador |
| Champs: |  Horacio Zeballos |
| Champd: |  Marcelo Demoliner / João Souza |
| After Year: | 2017 |
The 2014 Quito Challenger was a professional tennis tournament played on clay courts. It was the 20th edition of the tournament which was part of the 2014 ATP Challenger Tour. It took place in Quito, Ecuador between 15 and 21 September 2014.
| Country | Player | Rank1 | Seed | |
|---|---|---|---|---|
 Dominican Republic | Víctor Estrella Burgos | 69 | 1 | |
| Brazil | João Souza | 103 | 2 | |
| Argentina | Horacio Zeballos | 112 | 3 | |
| Argentina | Guido Pella | 158 | 4 | |
| Austin Krajicek | 164 | 5 | ||
| Chase Buchanan | 168 | 6 | ||
| Argentina | Juan Ignacio Londero | 186 | 7 | |
| Nikola Mektić | 230 | 8 |
The following players received wildcards into the singles main draw:
Chase Buchanan
Julio César Campozano Gonzalo Escobar David KonstantinovThe following player entered into the singles main draw with a protected ranking:
The following players received entry from the qualifying draw:
See main article: 2014 Quito Challenger – Singles.
Horacio Zeballos def. Nicolás Jarry, 6–4, 7–6(11–9)See main article: 2014 Quito Challenger – Doubles.
Marcelo Demoliner / João Souza def. 
Duilio Beretta / Martín Cuevas, 6–4, 6–4