| Date: | 28 April – 4 May |
| Edition: | 11th |
| Draw: | 32S / 16D |
| Surface: | Clay |
| Prize Money: | €85,000+H |
| Location: | Ostrava, Czech Republic |
| Champs: |  Andrey Kuznetsov |
| Champd: | Andrey Kuznetsov /  Adrián Menéndez Maceiras |
The 2014 Prosperita Open was a professional tennis tournament played on clay courts. It was the eleventh edition of the tournament which was part of the 2014 ATP Challenger Tour. It took place in Ostrava, Czech Republic between 28 April and 4 May 2014.
| Country | Player | Rank | Seed | |
|---|---|---|---|---|
 Czech Republic | Radek Štěpánek | 41 | 1 | |
 France | Stéphane Robert | 89 | 2 | |
| Spain | Pere Riba | 95 | 3 | |
 Slovenia | Blaž Rola | 111 | 4 | |
 Austria | Andreas Haider-Maurer | 113 | 5 | |
| Czech Republic | Jan Hájek | 125 | 6 | |
 Bosnia and Herzegovina | Damir Džumhur | 130 | 7 | |
| Russian Federation | Andrey Kuznetsov | 131 | 8 |
The following players received wildcards into the singles main draw:
Radek Štěpánek Jakub Lustyk Jan Minář Adam PavlásekThe following players received entry from the qualifying draw:
| Country | Player | Country | Player | Rank | Seed | |
|---|---|---|---|---|---|---|
| Philipp Marx |  Slovakia | Michal Mertiňák | 176 | 1 | ||
 Italy | Riccardo Ghedin | Italy | Claudio Grassi | 275 | 2 | |
| Spain | Gerard Granollers | Spain | Pere Riba | 437 | 3 | |
| Slovakia | Andrej Martin | Czech Republic | Jaroslav Pospíšil | 572 | 4 |
The following pairs received wildcards into the doubles main draw:
Marek Michalička / Jan Minář Jakub Lustyk / David Novak Dominik Kellovsky / Pavel Šnobel Andrey Kuznetsov def. Miloslav Mečíř Jr., 2–6, 6–3, 6–0 Andrey Kuznetsov / Adrián Menéndez Maceiras def. Alessandro Motti / Matteo Viola, 4–6, 6–3, [10–8]