The 2014 Prague Open was a professional tennis tournament played on clay courts. It was the 1st edition of the tournament which was part of the 2014 ATP Challenger Tour. It took place in Prague, Czech Republic between 9 and 15 June 2014.
| Country | Player | Rank1 | Seed | |
|---|---|---|---|---|
 Czech Republic | Lukáš Rosol | 52 | 1 | |
| Czech Republic | Jiří Veselý | 81 | 2 | |
 Kazakhstan | Aleksandr Nedovyesov | 101 | 3 | |
 Canada | Peter Polansky | 135 | 4 | |
| Czech Republic | Jan Hájek | 159 | 5 | |
 Spain | Adrián Menéndez Maceiras | 174 | 6 | |
 Italy | Matteo Viola | 184 | 7 | |
| Spain | Roberto Carballés Baena | 185 | 8 |
The following players received wildcards into the singles main draw:
Jakub Filipsky Adam Pavlásek Lukáš Rosol Pavel StaubertThe following players received entry from the qualifying draw:
| Country | Player | Country | Player | Rank1 | Seed | |
|---|---|---|---|---|---|---|
| Czech Republic | František Čermák |  Russian Federation | Michail Elgin | 123 | 1 | |
 Ireland | James Cluskey | Czech Republic | Jaroslav Pospíšil | 336 | 2 | |
| Vahid Mirzadeh | Canada | Peter Polansky | 386 | 3 | ||
| Czech Republic | Lukáš Dlouhý | Czech Republic | Jaroslav Levinský | 411 | 4 |
The following pairs received wildcards into the doubles main draw:
Marek Jaloviec / Daniel Knoflicek Jakub Filipsky / Pavel Staubert Tomáš Papik / Patrik RiklSee main article: 2014 Prague Open – Singles.
Lukáš Rosol def. Jiří Veselý, 3–6, 6–4, 6–4See main article: 2014 Prague Open – Doubles.
Roman Jebavý / Jiří Veselý def. Lee Hsin-han / 
Zhang Ze, 6–1, 6–3