| Date: | 4–9 November |
| Edition: | 11th |
| Surface: | Hard |
| Location: | Knoxville, United States |
| Champs: |  Adrian Mannarino |
| Champd: | Miķelis Lībietis /  Hunter Reese |
The 2014 Knoxville Challenger was a professional tennis tournament played on indoor hard courts. It was the eleventh edition of the tournament, which was part of the 2014 ATP Challenger Tour. It took place in Knoxville, United States between 4 and 9 November 2014.
| Country | Player | Rank1 | Seed | |
|---|---|---|---|---|
| Malek Jaziri | 71 | 1 | ||
| Adrian Mannarino | 73 | 2 | ||
| Sam Groth | 82 | 3 | ||
| Blaž Rola | 88 | 4 | ||
| Tim Smyczek | 95 | 5 | ||
| Michael Russell | 129 | 6 | ||
| Gastão Elias | 132 | 7 | ||
| Denis Kudla | 133 | 8 |
The following players received wildcards into the singles main draw:
The following player received entry as a special exemption into the singles main draw:
The following players received entry as an alternate into the singles main draw:
The following players received entry from the qualifying draw:
The following players received entry into the singles main draw as lucky losers:
See main article: 2014 Knoxville Challenger – Singles.
Adrian Mannarino def. 
Sam Groth 3–6, 7–6(8–6), 6–4[1]See main article: 2014 Knoxville Challenger – Doubles.
] / Hunter Reese def. Gastão Elias / 
Sean Thornley 6–3, 6–4[2]