The 2014 Karshi Challenger was a professional tennis tournament played on hard courts. It was the eighth edition of the tournament which was part of the 2014 ATP Challenger Tour. It took place in Qarshi, Uzbekistan between 19 and 24 May 2014.
| Country | Player | Rank1 | Seed | |
|---|---|---|---|---|
 Russian Federation | Alexander Kudryavtsev | 189 | 1 | |
 Canada | Filip Peliwo | 223 | 2 | |
| Russian Federation | Konstantin Kravchuk | 259 | 3 | |
 Japan | Yasutaka Uchiyama | 263 | 4 | |
| Chase Buchanan | 265 | 5 | ||
| Huang Liang-chi | 276 | 6 | ||
| Japan | Shuichi Sekiguchi | 281 | 7 | |
 Serbia | Peđa Krstin | 282 | 8 |
The following players received wildcards into the singles main draw:
Karen Khachanov
Shonigmatjon Shofayziyev Sanjar Fayziev Temur IsmailovThe following players received entry from the qualifying draw:
Denys Molchanov Anton Zaitcev Mikhail Ledovskikh
Laurent Rochette| Country | Player | Country | Player | Rank1 | Seed | |
|---|---|---|---|---|---|---|
| Russian Federation | Victor Baluda | Russian Federation | Konstantin Kravchuk | 244 | 1 | |
 India | Sriram Balaji | Chen Ti | 338 | 2 | ||
 Ireland | James Cluskey | India | Saketh Myneni | 348 | 3 | |
| Huang Liang-chi | India | Divij Sharan | 353 | 4 |
The following pairs received wildcards into the doubles main draw:
Shonigmatjon Shofayziyev / Vaja Uzakov Omad Boboqulov / Mikhail Esipov Sanjar Fayziev / Temur Ismailov
Nikoloz Basilashvili def. 
Chase Buchanan, 7–6(7–2), 6–2
Sergey Betov / Aliaksandr Bury def. 
Gong Maoxin / Peng Hsien-yin, 7–5, 1–6, [10–6]