| Date: | 25–30 March |
| Edition: | 4th |
| Draw: | 32S / 16D |
| Surface: | Hard |
| Prize Money: | $100,000+H |
| Location: | Guadalajara, Mexico |
| Champs: |  Gilles Müller |
| Champd: |  César Ramírez / Miguel Ángel Reyes-Varela |
The 2014 Jalisco Open was a professional tennis tournament played on hard courts. It was the fourth edition of the tournament which was part of the 2014 ATP Challenger Tour. It took place in Guadalajara, Mexico between 25 and 30 March 2014.
| Country | Player | Rank1 | Seed | |
|---|---|---|---|---|
 France | Kenny de Schepper | 66 | 1 | |
 Australia | Matthew Ebden | 67 | 2 | |
 Russian Federation | Alex Bogomolov Jr. | 94 | 3 | |
 Dominican Republic | Víctor Estrella Burgos | 100 | 4 | |
| Dustin Brown | 102 | 5 | ||
| Jan-Lennard Struff | 104 | 6 | ||
 Slovakia | Lukáš Lacko | 105 | 7 | |
| Peter Gojowczyk | 111 | 8 |
The following players received wildcards into the singles main draw:
Lucas Gómez Eduardo Yahir Orozco Rangel César Ramírez Miguel Ángel Reyes-VarelaThe following players gained entry into the singles main draw as a special exempt:
The following players gained entry into the singles main draw as an alternate:
The following players received entry from the qualifying draw:
See main article: 2014 Jalisco Open – Singles.
Gilles Müller def. 
Denis Kudla, 6–2, 6–2See main article: 2014 Jalisco Open – Doubles.
César Ramírez / Miguel Ángel Reyes-Varela def. Andre Begemann / Matthew Ebden, 6–4, 6–2
