The 2014 Claro Open Cali was a professional tennis tournament played on clay courts. It was the first edition of the tournament which was part of the 2014 ATP Challenger Tour. It took place in Cali, Colombia between 29 September and 4 October 2014.
| Country | Player | Rank1 | Seed | |
|---|---|---|---|---|
 Colombia | Alejandro Falla | 73 | 1 | |
 Italy | Paolo Lorenzi | 75 | 2 | |
 Dominican Republic | Víctor Estrella Burgos | 81 | 3 | |
 Argentina | Diego Sebastián Schwartzman | 88 | 4 | |
| Colombia | Alejandro González | 93 | 5 | |
| Argentina | Horacio Zeballos | 96 | 6 | |
 Brazil | João Souza | 101 | 7 | |
| Argentina | Facundo Bagnis | 122 | 8 |
The following players received wildcards into the singles main draw:
Nicolás Barrientos Eduardo Struvay
David Konstantinov José HernándezThe following players received entry from the qualifying draw:
Wilson Leite Marcelo DemolinerThe following player received entry by a special exempt:
The following players received entry as an alternate:
See main article: 2014 Claro Open Cali – Singles.
Paolo Lorenzi def. Víctor Estrella Burgos, 4–6, 6–3, 6–3See main article: 2014 Claro Open Cali – Doubles.
Guido Andreozzi / Guillermo Durán def. Alejandro González / 
César Ramírez, 6–3, 6–4