| Date: | 10 November – 16 November |
| Edition: | 10th |
| Surface: | Clay |
| Location: | Guayaquil, Ecuador |
| Champs: | Pablo Cuevas |
| Champd: |  Máximo González / Guido Pella |
The 2014 Challenger Ciudad de Guayaquil was a professional tennis tournament played on clay courts. It was the tenth edition of the tournament which is part of the 2014 ATP Challenger Tour. It took place in Guayaquil, Ecuador between November 10 and November 16, 2014.
| Country | Player | Rank1 | Seed | |
|---|---|---|---|---|
| Pablo Cuevas | 35 | 1 | ||
 Italy | Paolo Lorenzi | 70 | 2 | |
| Argentina | Diego Schwartzman | 78 | 3 | |
 Colombia | Alejandro González | 79 | 4 | |
 Brazil | João Souza | 91 | 5 | |
| Argentina | Horacio Zeballos | 100 | 6 | |
| Argentina | Máximo González | 101 | 7 | |
| Argentina | Facundo Bagnis | 113 | 8 |
The following players received wildcards into the singles main draw:
Gonzalo Escobar Diego Quiróz Giovanni LapenttiThe following players received entry from the qualifying draw:
The following player received entry by a lucky loser spot:
See main article: 2014 Challenger Ciudad de Guayaquil – Singles.
Paolo Lorenzi by walkoverSee main article: 2014 Challenger Ciudad de Guayaquil – Doubles.
Máximo González / Guido Pella def. 
Pere Riba / Jordi Samper-Montaña, 2–6, 7–6(7–3), [10–5]
