| Champ: |  Serena Williams |
| Runner: |  Victoria Azarenka |
| Score: | 6–4, 7–5 |
| Draw: | 28 |
| Seeds: | 8 |
See main article: 2014 Brisbane International.
Serena Williams was the defending champion, and successfully defended her title, defeating Victoria Azarenka in the final, 6–4, 7–5. Williams became the first woman to defend her title at the event.
The top two seeds receive a bye into the second round.
See also: 1. Serena Williams (champion)
See also: 2. Victoria Azarenka (final)
See also: 3. 
Maria Sharapova (semifinals)
See also: 4. 
Jelena Janković (semifinals)
See also: 5. Angelique Kerber (quarterfinals)
See also: 6. 
Caroline Wozniacki (withdrew because of a right shoulder injury)
See also: 7. Sabine Lisicki (second round, withdrew because of a gastrointestinal illness)
See also: 8. 
Carla Suárez Navarro (second round)
See also: 9. 
Dominika Cibulková (quarterfinals)