The 2013 UniCredit Czech Open was a professional tennis tournament played on clay courts. It was the 20th edition of the tournament that was part of the 2013 ATP Challenger Tour. It took place in Prostějov, Czech Republic between 3 and 9 June 2013.
| Country | Player | Rank1 | Seed | |
|---|---|---|---|---|
| Florian Mayer | 30 | 1 | ||
 Czech Republic | Lukáš Rosol | 36 | 2 | |
 Finland | Jarkko Nieminen | 38 | 3 | |
 Spain | Albert Montañés | 47 | 4 | |
| Czech Republic | Radek Štěpánek | 52 | 5 | |
| Spain | Albert Ramos | 64 | 6 | |
| Spain | Guillermo García López | 79 | 7 | |
| Czech Republic | Jan Hájek | 87 | 8 |
The following players received wildcards into the singles main draw:
The following player received entry using a protected ranking:
Víctor EstrellaThe following players received entry as alternates into the singles main draw:
Damir Džumhur Dušan Lojda Jordi Samper-Montaña
Marek SemjanThe following players received entry from the qualifying draw:
The following player received entry as a lucky loser:
Michal Konečný| Country | Player | Country | Player | Rank1 | Seed | |
|---|---|---|---|---|---|---|
| Nicholas Monroe | Simon Stadler | 164 | 1 | |||
| Rik de Voest | Raven Klaasen | 198 | 2 | |||
 Poland | Mateusz Kowalczyk | Czech Republic | Lukáš Rosol | 232 | 3 | |
| Czech Republic | Jaroslav Pospíšil | Slovakia | Igor Zelenay | 260 | 4 |
The following pairs received wildcards into the doubles main draw:
Marek Jaloviec / Václav Šafránek Michal Konečný / Adam Pavlásek Jaroslav Levinský / Dušan LojdaThe following pair received entry as an alternate:
Dominik Kellovský / Robert Rumler Radek Štěpánek def. Jiří Veselý, 6–4, 6–2
Nicholas Monroe / Simon Stadler def. Mateusz Kowalczyk / Lukáš Rosol, 6–4, 6–4