| Date: | 13 – 19 May |
| Edition: | 4th |
| Location: | Prague, Czech Republic |
| Venue: | TK Sparta Prague |
| Champs: |  Lucie Šafářová |
| Champd: | Renata Voráčová / Barbora Záhlavová-Strýcová |
The 2013 Sparta Prague Open was a professional tennis tournament played on outdoor clay courts. It was the fourth edition of the tournament which was part of the 2013 ITF Women's Circuit, offering a total of $100,000 in prize money. It took place in Prague, Czech Republic, from 13 to 19 May 2013.
| Country | Player | Rank1 | Seed | |
|---|---|---|---|---|
| Czech Republic | Klára Zakopalová | 20 | 1 | |
| Czech Republic | Lucie Šafářová | 25 | 2 | |
| Czech Republic | Lucie Hradecká | 57 | 3 | |
| Annika Beck | 59 | 4 | ||
 Sweden | Johanna Larsson | 66 | 5 | |
 Slovakia | Jana Čepelová | 83 | 6 | |
| Monica Puig | 88 | 7 | ||
 Japan | Misaki Doi | 90 | 8 |
The following players received wildcards into the singles main draw:
The following players received entry from the qualifying draw:
The following player received entry by a Special Exempt:
See main article: 2013 Sparta Prague Open – Singles.
Lucie Šafářová def. 
Alexandra Cadanțu 3–6, 6–1, 6–1See main article: 2013 Sparta Prague Open – Doubles.
Renata Voráčová / Barbora Záhlavová-Strýcová def. 
Irina Falconi / Eva Hrdinová 6–4, 6–0