| Date: | 9–15 September |
| Edition: | 6th |
| Surface: | Clay |
| Location: | Cali, Colombia |
| Champs: |  Facundo Bagnis |
| Champd: | Guido Andreozzi / Eduardo Schwank |
The 2013 Seguros Bolívar Open Cali was a professional tennis tournament played on clay courts. It was the sixth edition of the tournament which was part of the 2013 ATP Challenger Tour. It took place in Cali, Colombia between 9 and 15 September 2013.
| Country | Player | Rank1 | Seed | |
|---|---|---|---|---|
 Brazil | João Souza | 121 | 1 | |
| Argentina | Guido Andreozzi | 160 | 2 | |
| Argentina | Agustín Velotti | 170 | 3 | |
| Argentina | Facundo Bagnis | 180 | 4 | |
| Argentina | Facundo Argüello | 208 | 5 | |
| Brazil | Fabiano de Paula | 228 | 6 | |
| Brazil | Ricardo Hocevar | 260 | 7 | |
 Colombia | Carlos Salamanca | 270 | 8 |
The following players received wildcards into the singles main draw:
Giovanni Lapentti Alvaro Ochoa Eduardo Struvay Carlos SalamancaThe following players received entry from the qualifying draw:
Juan Ignacio Londero
Duilio Beretta Sebastian Exequiel Pini Fernando RomboliSee main article: 2013 Seguros Bolívar Open Cali – Singles.
Facundo Bagnis def. Facundo Argüello 6–2, 4–6, 6–3See main article: 2013 Seguros Bolívar Open Cali – Doubles.
Guido Andreozzi / Eduardo Schwank def. Carlos Salamanca / João Souza 6–2, 6–4