The 2013 San Luis Open Challenger was a professional tennis tournament played on clay courts. It was the 20th edition of the tournament which was part of the 2013 ATP Challenger Tour. It took place in San Luis Potosí, Mexico between 25 and 31 March 2013.
| Country | Player | Rank1 | Seed | |
|---|---|---|---|---|
 Australia | John Millman | 130 | 1 | |
 France | Jonathan Dasnières de Veigy | 147 | 2 | |
 Argentina | Diego Sebastián Schwartzman | 149 | 3 | |
| Marco Chiudinelli | 151 | 4 | ||
 Tunisia | Malek Jaziri | 163 | 5 | |
| Peter Gojowczyk | 170 | 6 | ||
 Slovakia | Andrej Martin | 173 | 7 | |
| Argentina | Agustín Velotti | 182 | 8 |
The following players received wildcards into the singles main draw:
The following players received entry as an alternate into the singles main draw:
The following players received entry from the qualifying draw:
| Country | Player | Country | Player | Rank1 | Seed | |
|---|---|---|---|---|---|---|
 India | Purav Raja | India | Divij Sharan | 246 | 1 | |
| Lee Hsin-han | Peng Hsien-yin | 258 | 2 | |||
 Brazil | Marcelo Demoliner | Franco Škugor | 276 | 3 | ||
| Marin Draganja |  Spain | Adrián Menéndez Maceiras | 307 | 4 |
The following pairs received wildcards into the doubles main draw:
Miguel Gallardo Valles / Nicolás Massú Daniel Garza / Miguel Ángel Reyes-Varela
Riccardo Ghedin / Manuel SánchezSee main article: 2013 San Luis Open Challenger – Singles.
Alessio di Mauro def. 
Daniel Kosakowski, 4–6, 6–3, 6–2See main article: 2013 San Luis Open Challenger – Doubles.
Adrián Menéndez Maceiras def. Marco Chiudinelli / Peter Gojowczyk, 6–4, 6–3