The 2013 Roma Open was a professional tennis tournament played on clay courts. It was the twelfth edition of the tournament which was part of the 2013 ATP Challenger Tour. It took place in Rome, Italy between 6 and 12 May 2013.
| Country | Player | Rank1 | Seed | |
|---|---|---|---|---|
 Spain | Albert Montañés | 84 | 1 | |
 Slovenia | Aljaž Bedene | 85 | 2 | |
| Slovenia | Blaž Kavčič | 97 | 3 | |
 Argentina | Guido Pella | 102 | 4 | |
 France | 103 | 5 | ||
 Austria | Andreas Haider-Maurer | 105 | 6 | |
| France | Adrian Mannarino | 109 | 7 | |
| Argentina | Federico Delbonis | 112 | 8 | |
 Belgium | Steve Darcis | 115 | 9 |
The following players received wildcards into the singles main draw:
The following players received entry as a special exempt into the singles main draw:
The following players received entry from the qualifying draw:
The following players received entry as lucky losers:
Denys Mylokostov| Country | Player | Country | Player | Rank1 | Seed | |
|---|---|---|---|---|---|---|
| Andre Begemann | Martin Emmrich | 122 | 1 | |||
| Philipp Marx |  Romania | Florin Mergea | 142 | 2 | ||
| Nicholas Monroe | Simon Stadler | 159 | 3 | |||
 Israel | Andy Ram |  Brazil | André Sá | 176 | 4 |
The following pairs received wildcards into the doubles main draw:
The following pair received entry using a protected ranking:
The following pair received entry as an alternate into the doubles main draw:
Aljaž Bedene def. 
Filippo Volandri, 6–4, 6–2 Florin Mergea, 7–6(7–4), 6–3
