| Date: | 29 April – 5 May |
| Edition: | 10th |
| Draw: | 32S / 16D |
| Surface: | Clay |
| Prize Money: | €85,000+H |
| Location: | Ostrava, Czech Republic |
| Champs: |  Jiří Veselý |
| Champd: |  Steve Darcis / Olivier Rochus |
The 2013 Prosperita Open was a professional tennis tournament played on clay courts. It was the tenth edition of the tournament which was part of the 2013 ATP Challenger Tour. It took place in Ostrava, Czech Republic between 29 April and 5 May 2013.
| Country | Player | Rank1 | Seed | |
|---|---|---|---|---|
| Czech Republic | Lukáš Rosol | 48 | 1 | |
 Slovenia | Aljaž Bedene | 82 | 2 | |
 France | Guillaume Rufin | 88 | 3 | |
| Czech Republic | Jan Hájek | 95 | 4 | |
 Austria | Andreas Haider-Maurer | 111 | 5 | |
| Belgium | Steve Darcis | 116 | 6 | |
| Belgium | Olivier Rochus | 145 | 7 | |
 Belarus | Uladzimir Ignatik | 147 | 8 |
The following players received wildcards into the singles main draw:
Tomáš Papík Adam Pavlásek Lukáš RosolThe following players received entry from the qualifying draw:
| Country | Player | Country | Player | Rank1 | Seed | |
|---|---|---|---|---|---|---|
 Poland | Tomasz Bednarek | Poland | Mateusz Kowalczyk | 192 | 1 | |
| Czech Republic | Jaroslav Pospíšil |  Slovakia | Igor Zelenay | 225 | 2 | |
| Aljaž Bedene | Marin Draganja | 300 | 3 | |||
| Nikola Mektić | Franko Škugor | 362 | 4 |
The following pairs received wildcards into the doubles main draw:
Dominik Kellovský / David Poljak Jaroslav Levinský / Ivo Minář Adam Pavlásek / Jiří VeselýThe following pairs received entry as an alternate:
Victor Crivoi / Jonathan Eysseric
Adam El-Effendi / 
Darren Walsh Jiří Veselý def. Steve Darcis, 6–4, 6–4 Steve Darcis / Olivier Rochus def. Tomasz Bednarek / Mateusz Kowalczyk, 7–5, 7–5