The 2013 Prague Open by Advantage Cars was a professional tennis tournament played on clay courts. It was the 20th edition of the tournament which was part of the 2013 ATP Challenger Tour. It took place in Prague, Czech Republic between 10 and 16 June 2013.
| Country | Player | Rank1 | Seed | |
|---|---|---|---|---|
 Austria | Andreas Haider-Maurer | 105 | 1 | |
 Italy | Filippo Volandri | 111 | 2 | |
| Wayne Odesnik | 113 | 3 | ||
 Spain | Rubén Ramírez Hidalgo | 123 | 4 | |
| Italy | Matteo Viola | 132 | 5 | |
| Spain | Daniel Muñoz de la Nava | 145 | 6 | |
 Ukraine | Oleksandr Nedovyesov | 160 | 7 | |
 Argentina | Facundo Argüello | 170 | 8 |
The following players received wildcards into the singles main draw:
Dušan Lojda Ivo Minář
Adrian Sikora Robin StaněkThe following players received entry a special exempt into the singles main draw:
The following players received entry from the qualifying draw:
Víctor Estrella Lukáš Maršoun Jan Šátral Dominik Süč| Country | Player | Country | Player | Rank1 | Seed | |
|---|---|---|---|---|---|---|
 Poland | Mateusz Kowalczyk |  Sweden | Andreas Siljeström | 228 | 1 | |
| Lee Hsin-han | Peng Hsien-yin | 246 | 2 | |||
 Australia | Colin Ebelthite | Australia | Rameez Junaid | 253 | 3 | |
| Mate Pavić | Franko Škugor | 300 | 4 |
The following pair received a wildcard into the doubles main draw:
Jakub Filipský / Kryštof JánošíkThe following pair received entry using a protected ranking:
The following pair received entry as an alternate into the doubles main draw:
See main article: 2013 Prague Open – Singles.
Oleksandr Nedovyesov def. Javier Martí, 6–0, 6–1See main article: 2013 Prague Open – Doubles.
Vahid Mirzadeh / Denis Zivkovic, 6–4, 4–6, [10–5]