| Date: | 28 October–3 November |
| Edition: | 1st |
| Category: | WTA 125K series |
| Surface: | Hard |
| Prize Money: | $125,000 |
| Location: | Nanjing, China |
| Champs: |  Zhang Shuai |
| Champd: |  Misaki Doi / Xu Yifan |
The 2013 Nanjing Ladies Open was a professional tennis tournament played on outdoor hard courts. It was the inaugural edition of the tournament and was part of the 2013 WTA 125K series. It took place in Nanjing, China, on 28 October–3 November 2013.[1]
| Country | Player | Rank1 | Seed |
|---|---|---|---|
| Japan | Kimiko Date-Krumm | 55 | 1 |
 Belgium | Yanina Wickmayer | 60 | 2 |
| China | Zhang Shuai | 61 | 3 |
| Japan | Ayumi Morita | 62 | 4 |
 France | Caroline Garcia | 72 | 5 |
| Japan | Misaki Doi | 78 | 6 |
| Ajla Tomljanović | 79 | 7 | |
 Slovakia | Anna Karolina Schmiedlova | 81 | 8 |
The following players received wildcards into the singles main draw:
The following players received entry from the qualifying draw:
The following player received entry into the singles main draw as a lucky loser:
| Country | Player | Country | Player | Rank | Seed | |
|---|---|---|---|---|---|---|
 Kazakhstan | Yaroslava Shvedova | China | Zhang Shuai | 122 | 1 | |
| Petra Martić | China | Zheng Saisai | 130 | 2 | ||
 Ukraine | Irina Buryachok |  Poland | Katarzyna Piter | 149 | 3 | |
 Romania | Irina-Camelia Begu | Belgium | Yanina Wickmayer | 190 | 4 |
See main article: 2013 Nanjing Ladies Open – Singles.
Zhang Shuai def. Ayumi Morita 6–4, retiredSee main article: 2013 Nanjing Ladies Open – Doubles.
Misaki Doi / Xu Yifan def. Yaroslava Shvedova / Zhang Shuai 6–1, 6–4
