| Date: | 4–10 November |
| Edition: | 10th |
| Surface: | Hard |
| Location: | Knoxville, United States |
| Champs: |  Tim Smyczek |
| Champd: |  Samuel Groth / John-Patrick Smith |
The 2013 Knoxville Challenger was a professional tennis tournament played on hard courts. It was the tenth edition of the tournament which was part of the 2013 ATP Challenger Tour. It took place in Knoxville, United States between 4 and 10 November 2013.
| Country | Player | Rank1 | Seed | |
|---|---|---|---|---|
| Tim Smyczek | 83 | 1 | ||
| Denis Kudla | 90 | 2 | ||
| Jack Sock | 94 | 3 | ||
| Michael Russell | 96 | 4 | ||
| Donald Young | 104 | 5 | ||
| Ryan Harrison | 105 | 6 | ||
| Rajeev Ram | 120 | 7 | ||
| Alex Kuznetsov | 121 | 8 |
The following players received wildcards into the singles main draw:
Jarryd Chaplin Jarmere Jenkins Jack SockThe following players used protected ranking into the singles main draw:
Laurent RochetteThe following players received entry as an alternate into the singles main draw:
The following players received entry from the qualifying draw:
Mitchell Frank Kevin King
Sanam SinghThe following players received entry into the singles main draw as lucky losers:
See main article: 2013 Knoxville Challenger – Singles.
Tim Smyczek def. 
Peter Polansky 6–4, 6–2See main article: 2013 Knoxville Challenger – Doubles.
Samuel Groth / John-Patrick Smith def. Carsten Ball / Peter Polansky 6–7(6–8), 6–2, [10–7]