The 2013 Karshi Challenger was a professional tennis tournament played on hard courts. It was the seventh edition of the tournament which was part of the 2013 ATP Challenger Tour. It took place in Qarshi, Uzbekistan between 6 and 12 May 2013.
| Country | Player | Rank1 | Seed | |
|---|---|---|---|---|
 Russian Federation | Teymuraz Gabashvili | 177 | 1 | |
 Israel | Amir Weintraub | 183 | 2 | |
| Russian Federation | Konstantin Kravchuk | 187 | 3 | |
 Ukraine | Oleksandr Nedovyesov | 194 | 4 | |
 Czech Republic | Jan Mertl | 196 | 5 | |
| Chen Ti | 216 | 6 | ||
 Moldova, Republic of | Radu Albot | 246 | 7 | |
 Belarus | Dzmitry Zhyrmont | 269 | 8 |
The following players received wildcards into the singles main draw:
Sanjar Fayziev Sarvar Ikramov Temur Ismailov Nigmat ShofayzievThe following players received entry from the qualifying draw:
| Country | Player | Country | Player | Rank1 | Seed | |
|---|---|---|---|---|---|---|
| James Cerretani |  Canada | Adil Shamasdin | 163 | 1 | ||
 Australia | Jordan Kerr | Russian Federation | Konstantin Kravchuk | 248 | 2 | |
| Moldova, Republic of | Radu Albot | Ukraine | Oleksandr Nedovyesov | 435 | 3 | |
| Russian Federation | Teymuraz Gabashvili | Israel | Amir Weintraub | 597 | 4 |
The following pairs received wildcards into the doubles main draw:
Omad Boboqulov / Pavel Tsoy Sanjar Fayziev / Nigmat Shofayziev Sarvar Ikramov / Batyr Sapaev Teymuraz Gabashvili def. Radu Albot, 6–4, 6–4
Guillermo Olaso def. Jordan Kerr / Konstantin Kravchuk, 7–6(7–5), 7–5