| Champ: |  Serena Williams |
| Runner: |  Victoria Azarenka |
| Score: | 6–1, 6–3 |
| Draw: | 56 |
| Seeds: | 16 |
See main article: 2013 Italian Open (tennis).
Serena Williams defeated Victoria Azarenka in the final, 6–1, 6–3 to win the women's singles tennis title at the 2013 Italian Open. It was her second Internazionali BNL d'Italia title, and she won it without dropping a set, without dropping more than three games per set, and without dropping more than four games per match.
Maria Sharapova was the two-time defending champion, but withdrew in the quarterfinals against Sara Errani due to illness.
The top eight seeds receive a bye into the second round.
