The Women's 400 metres hurdles event at the 2013 European Athletics U23 Championships was held in Tampere, Finland, at Ratina Stadium on 12 and 13 July.
| Gold | Vera Rudakova | |
| Silver | Bianca Baak | |
| Bronze | Anastasiya Lebid |
13 July 2013
| Rank | Name | Nationality | Lane | Reaction Time | Time | Notes | |||
|---|---|---|---|---|---|---|---|---|---|
| align=left | align=left | | 4 | 0.272 | 55.92 | PB | |||
| align=left | align=left | 5 | 0.235 | 56.75 | PB | ||||
| align=left | align=left | | 6 | 0.294 | 56.92 | ||||
| 4 | align=left | align=left |  | 3 | 0.211 | 57.13 | |||
| 5 | align=left | align=left |  | 2 | 0.223 | 57.27 | PB | ||
| 6 | align=left | align=left | | 8 | 0.194 | 58.24 | |||
| 7 | align=left | align=left | 1 | 0.243 | 58.52 | ||||
| 8 | align=left | align=left | | 7 | 0.200 | 58.55 |
Qualified: First 2 in each heat (Q) and 2 best performers (q) advance to the Final
| Rank | Name | Nationality | Time | Notes | |||
|---|---|---|---|---|---|---|---|
| 1 | align=left | align=left | | 57.15 | Q | ||
| 2 | align=left | align=left | | 57.40 | Q | ||
| 3 | align=left | align=left | 57.58 | Q PB | |||
| 4 | align=left | align=left | | 57.87 | q | ||
| 5 | align=left | align=left | | 58.20 | Q | ||
| 6 | align=left | align=left | | 58.54 | Q | ||
| 7 | align=left | align=left | | 58.94 | Q | ||
| 8 | align=left | align=left | 59.11 | q | |||
| 9 | align=left | align=left | | 59.31 | |||
| 10 | align=left | align=left |  | 59.45 | SB | ||
| 10 | align=left | align=left |  | 59.45 | |||
| 12 | align=left | align=left | | 59.87 | |||
| 13 | align=left | align=left |  | 1:00.52 | |||
| 14 | align=left | align=left | | 1:00.53 | |||
| 15 | align=left | align=left | 1:01.66 | ||||
| 16 | align=left | align=left |  | 1:01.68 | |||
| 17 | align=left | align=left |  | 1:03.02 | |||
| align=left | align=left |  | DQ | R 162.7 |
12 July 2013 / 17:00
| Rank | Name | Nationality | Lane | Reaction Time | Time | Notes | |||
|---|---|---|---|---|---|---|---|---|---|
| 1 | align=left | align=left | | 7 | 0.200 | 57.40 | Q | ||
| 2 | align=left | align=left | 4 | 0.179 | 57.58 | Q PB | |||
| 3 | align=left | align=left | | 5 | 0.252 | 57.87 | q | ||
| 4 | align=left | align=left | | 2 | 0.264 | 1:00.52 | |||
| 5 | align=left | align=left | | 3 | 0.146 | 1:01.68 | |||
| align=left | align=left | | 6 | F1F2 | DQ R 162.7 |
12 July 2013 / 17:08
| Rank | Name | Nationality | Lane | Reaction Time | Time | Notes | |||
|---|---|---|---|---|---|---|---|---|---|
| 1 | align=left | align=left | | 4 | 0.315 | 58.20 | Q | ||
| 2 | align=left | align=left | | 7 | 0.213 | 58.94 | Q | ||
| 3 | align=left | align=left | | 2 | 0.215 | 59.31 | |||
| 4 | align=left | align=left | | 6 | 0.218 | 59.45 | SB | ||
| 5 | align=left | align=left | | 5 | 0.182 | 59.87 | |||
| 6 | align=left | align=left | 3 | 0.181 | 1:01.66 |
12 July 2013 / 17:16
| Rank | Name | Nationality | Lane | Reaction Time | Time | Notes | |||
|---|---|---|---|---|---|---|---|---|---|
| 1 | align=left | align=left | | 7 | 0.262 | 57.15 | Q | ||
| 2 | align=left | align=left | | 6 | 0.206 | 58.54 | Q | ||
| 3 | align=left | align=left | 2 | 0.178 | 59.11 | q | |||
| 4 | align=left | align=left | | 4 | 0.231 | 59.45 | |||
| 5 | align=left | align=left | | 5 | 0.255 | 1:00.53 | |||
| 6 | align=left | align=left | | 3 | 0.170 | 1:03.02 |
According to an unofficial count, 18 athletes from 13 countries participated in the event.