| Date: | 11 – 17 November |
| Edition: | 9th |
| Surface: | Clay |
| Location: | Guayaquil, Ecuador |
| Champs: |  Leonardo Mayer |
| Champd: | Stephan Fransen / Wesley Koolhof |
The 2013 Challenger Ciudad de Guayaquil was a professional clay-court tennis tournament. It was the ninth edition of the tournament, which was part of the 2013 ATP Challenger Tour. It took place in Guayaquil, Ecuador between 11 and 17 November 2013.
| Country | Player | Rank1 | Seed | |
|---|---|---|---|---|
| Argentina | Leonardo Mayer | 93 | 1 | |
 Italy | Paolo Lorenzi | 102 | 2 | |
| Argentina | Diego Sebastián Schwartzman | 115 | 3 | |
| Wayne Odesnik | 136 | 4 | ||
| Paul Capdeville | 148 | 5 | ||
| Gastão Elias | 155 | 6 | ||
| Argentina | Máximo González | 161 | 7 | |
 Dominican Republic | Víctor Estrella Burgos | 199 | 8 |
The following players received wildcards into the singles main draw:
Gonzalo Escobar Giovanni Lapentti Roberto Quiroz Jorman ReyesThe following players received entry from the qualifying draw:
See main article: 2013 Challenger Ciudad de Guayaquil – Singles.
Leonardo Mayer def. Pedro Sousa 6–4, 7–5See main article: 2013 Challenger Ciudad de Guayaquil – Doubles.
Roman Borvanov / Alexander Satschko 1–6, 6–2, [10–5]