| Date: | 14–20 May |
| Edition: | 3rd |
| Surface: | Clay |
| Location: | Prague Czech Republic |
| Venue: | TK Sparta Prague |
| Champs: |  Lucie Šafářová |
| Champd: |  Alizé Cornet / Virginie Razzano |
The 2012 Sparta Prague Open was a professional tennis tournament played on clay courts. It was the third edition of the tournament which was part of the 2012 ITF Women's Circuit. It took place at the TK Sparta Prague in Prague, Czech Republic, from 14 to 20 May 2012.
| Country | Player | Rank1 | Seed | |
|---|---|---|---|---|
| Czech Republic | Lucie Šafářová | 23 | 1 | |
| Czech Republic | Klára Zakopalová | 44 | 2 | |
| Czech Republic | Barbora Záhlavová-Strýcová | 56 | 3 | |
 Hungary | Tímea Babos | 64 | 4 | |
| Eleni Daniilidou | 65 | 5 | ||
 United Kingdom | Elena Baltacha | 67 | 6 | |
 Japan | Kimiko Date-Krumm | 74 | 7 | |
| Japan | Ayumi Morita | 80 | 8 |
The following players received wildcards into the singles main draw:
Denisa Allertová
Jana Čepelová Martina Přádová Tereza SmitkováThe following players received entry from the qualifying draw:
The following players received entry into the singles main draw as lucky losers:
See main article: 2012 Sparta Prague Open – Singles.
Lucie Šafářová def. Klára Zakopalová 6–3, 7–5See main article: 2012 Sparta Prague Open – Doubles.
Alizé Cornet / Virginie Razzano def. 
Akgul Amanmuradova / 
Casey Dellacqua 6–2, 6–3