| Date: | 10–16 September |
| Edition: | 5th |
| Surface: | Clay |
| Location: | Cali, Colombia |
| Champs: |  João Souza |
| Champd: |  Juan Sebastián Cabal / Robert Farah |
The 2012 Seguros Bolívar Open Cali was a professional tennis tournament played on clay courts. It was the fifth edition of the tournament which was part of the 2012 ATP Challenger Tour. It took place in Cali, Colombia between 10 and 16 September 2012.
| Country | Player | Rank1 | Seed | |
|---|---|---|---|---|
| Colombia | Santiago Giraldo | 46 | 1 | |
| Colombia | Alejandro Falla | 51 | 2 | |
 Spain | Rubén Ramírez Hidalgo | 94 | 3 | |
| Wayne Odesnik | 136 | 4 | ||
| Brazil | Thiago Alves | 138 | 5 | |
 Argentina | Martín Alund | 140 | 6 | |
| Argentina | Guido Pella | 152 | 7 | |
| Brazil | João Souza | 155 | 8 |
The following players received wildcards into the singles main draw:
Facundo Bagnis Nicolás Barrientos Santiago Giraldo Felipe MantillaThe following players received entry from the qualifying draw:
Fabiano de Paula Juan Sebastián Gómez
Kevin Kim Sebastian SerranoSee main article: 2012 Seguros Bolívar Open Cali – Singles.
João Souza def. Thiago Alves, 6–2, 6–4See main article: 2012 Seguros Bolívar Open Cali – Doubles.
Juan Sebastián Cabal / Robert Farah def. Marcelo Demoliner / João Souza, 6–3, 7–6(7–4)