The 2012 Seguros Bolívar Open Barranquilla was a professional tennis tournament played on clay courts. It was the second edition of the tournament which was part of the 2012 ATP Challenger Tour. It took place in Barranquilla, Colombia between 26 March – 1 April 2012.
| Country | Player | Rank1 | Seed | |
|---|---|---|---|---|
 Colombia | Alejandro Falla | 71 | 1 | |
 Brazil | João Souza | 104 | 2 | |
 Argentina | Diego Junqueira | 112 | 3 | |
 France | Éric Prodon | 114 | 4 | |
| Paul Capdeville | 119 | 5 | ||
 Spain | Rubén Ramírez Hidalgo | 121 | 5 | |
| Argentina | Horacio Zeballos | 127 | 7 | |
 Italy | Matteo Viola | 154 | 8 |
The following players received wildcards into the singles main draw:
The following players received entry from the qualifying draw:
See main article: 2012 Seguros Bolívar Open Barranquilla – Singles.
Alejandro Falla def. Horacio Zeballos, 6–4, 6–1See main article: 2012 Seguros Bolívar Open Barranquilla – Doubles.
Nicholas Monroe / Maciek Sykut def. Marcel Felder / Frank Moser, 2–6, 6–3, [10–5]