The 2012 Samarkand Challenger was a professional tennis tournament played on clay courts. It was the 16th edition of the tournament which was part of the 2012 ATP Challenger Tour. It took place in Samarkand, Uzbekistan between 6 and 12 August 2012.
| Country | Player | Rank1 | Seed | |
|---|---|---|---|---|
 Russian Federation | Igor Kunitsyn | 143 | 1 | |
 Kazakhstan | 157 | 2 | ||
 Serbia | Dušan Lajović | 169 | 3 | |
 Slovakia | Kamil Čapkovič | 220 | 4 | |
 Uzbekistan | Farrukh Dustov | 235 | 5 | |
 Ukraine | Ivan Sergeyev | 237 | 6 | |
 Australia | Brydan Klein | 241 | 7 | |
| Slovakia | Andrej Martin | 260 | 8 |
The following players received wildcards into the singles main draw:
Sarvar Ikramov Temur Ismailov Sergey Shipilov Vaja UzakovThe following players received entry from the qualifying draw:
N.Sriram Balaji Adrian Sikora
Dzmitry ZhyrmontSee main article: 2012 Samarkand Challenger – Singles.
Dušan Lajović def. Farrukh Dustov, 6–3, 6–2See main article: 2012 Samarkand Challenger – Doubles.
Oleksandr Nedovyesov / Ivan Sergeyev def. Divij Sharan / Vishnu Vardhan, 6–4, 7–6(7–1)