The 2012 Roma Open was a professional tennis tournament played on clay courts. It was the eleventh edition of the tournament which was part of the 2012 ATP Challenger Tour. It took place in Rome, Italy between 7 and 12 May 2012.
| Country | Player | Rank1 | Seed | |
|---|---|---|---|---|
 Luxembourg | Gilles Müller | 62 | 1 | |
| Ernests Gulbis | 84 | 2 | ||
| Rui Machado | 90 | 3 | ||
 Italy | Simone Bolelli | 98 | 4 | |
| Antonio Veić | 127 | 5 | ||
 United Kingdom | James Ward | 137 | 6 | |
 France | Guillaume Rufin | 154 | 7 | |
 Russian Federation | Andrey Kuznetsov | 157 | 8 |
The following players received wildcards into the singles main draw:
Alberto Brizzi Marco Cecchinato Thomas Fabbiano Gianluigi QuinziThe following players received entry as a special exempt into the singles main draw:
The following players received entry from the qualifying draw:
See main article: 2012 Roma Open – Singles.
Jerzy Janowicz def. Gilles Müller, 7–6(7–3), 6–3See main article: 2012 Roma Open – Doubles.
Jamie Delgado / Ken Skupski def. 
Adrián Menéndez / Walter Trusendi, 6–1, 6–4
