The 2012 Rai Open was a professional tennis tournament played on clay courts. It was the fourth edition of the tournament which was part of the 2012 ATP Challenger Tour. It took place in Rome, Italy between 16 and 22 April 2012.
| Country | Player | Rank1 | Seed | |
|---|---|---|---|---|
| Ernests Gulbis | 78 | 1 | ||
 Spain | Guillermo García López | 80 | 2 | |
| Rui Machado | 97 | 3 | ||
| Igor Sijsling | 120 | 4 | ||
| Grega Žemlja | 126 | 5 | ||
 France | Adrian Mannarino | 127 | 6 | |
 Turkey | Marsel İlhan | 128 | 7 | |
| Thomas Schoorel | 131 | 8 |
The following players received wildcards into the singles main draw:
Nicola Barraco Marco Cecchinato Gianluca Naso Vincenzo SantopadreThe following players received entry from the qualifying draw:
See main article: 2012 Rai Open – Singles.
Roberto Bautista Agut def. Rui Machado, 6–7 (7–9), 6–4, 6–3See main article: 2012 Rai Open – Doubles.
Jonathan Marray def. 
Andrei Dăescu / Florin Mergea, 6–4, 7–6(7–0)
