The 2010 Zagreb Open was a professional tennis tournament played on outdoor red clay courts. It was part of the 2010 ATP Challenger Tour. It took place in Zagreb, Croatia between May 10 and May 16, 2010.
| Nationality | Player | Ranking* | Seeding | |
|---|---|---|---|---|
 Brazil | Marcos Daniel | 91 | 1 | |
| Nicolás Massú | 92 | 2 | ||
| Blaž Kavčič | 101 | 3 | ||
 Austria | Daniel Köllerer | 106 | 4 | |
 India | Somdev Devvarman | 109 | 5 | |
 Spain | Rubén Ramírez Hidalgo | 119 | 6 | |
| Spain | Santiago Ventura Bertomeu | 137 | 7 | |
| Grega Žemlja | 140 | 8 |
The following players received wildcards into the singles main draw:
The following players received entry from the qualifying draw:
The following player received special exempt into the main draw:
See main article: 2010 Zagreb Open – Singles. 
Yuri Schukin def. Santiago Ventura, 6–3, 7–5
See main article: 2010 Zagreb Open – Doubles. Andre Begemann / 
Matthew Ebden def. Rubén Ramírez Hidalgo / Santiago Ventura, 7–6(5), 5–7, [10–3]
