| Score: | 6–4, 7–6(7–5) |
| Draw: | 48 (5) |
| Seeds: | 16 |
See main article: 2010 Wimbledon Championships. Mark Knowles and Anna-Lena Grönefeld were the defending champions but Grönefeld did not compete. Knowles partnered with Katarina Srebotnik but they lost in the third round to Paul Hanley and Chan Yung-jan.
Leander Paes and Cara Black defeated Wesley Moodie and Lisa Raymond in the final, 6–4, 7–6(7–5) to win the mixed doubles tennis title at the 2010 Wimbledon Championships.[1]
All seeds received a bye into the second round.
See also: 1 and 1. 
Nenad Zimonjić / 
Samantha Stosur (third round)
See also: 2 and 4. 
Leander Paes / Cara Black (champions)
See also: 3 and 3. Mahesh Bhupathi / 
Liezel Huber (second round)
See also: 4 and 2. 
Oliver Marach / 
Nuria Llagostera Vives (second round)
See also: 5 and 4. Mark Knowles / Katarina Srebotnik (third round)
See also: 6 and 1. 
Daniel Nestor / Bethanie Mattek-Sands (third round)
See also: 7 and 2. 
Max Mirnyi / 
Alisa Kleybanova (third round)
See also: 8 and 3. 
Mariusz Fyrstenberg / 
Yan Zi (third round)
See also: 9 and 3. 
Lukáš Dlouhý / Iveta Benešová (semifinals)
See also: 10 and 1. 
Marcelo Melo / Rennae Stubbs (semifinals)
See also: 11 and 2. Wesley Moodie / Lisa Raymond (final)
See also: 12 and 4. Paul Hanley / Chan Yung-jan (quarterfinals)
See also: 13 and 2. 
Robert Lindstedt / Ekaterina Makarova (second round)
See also: 14. Marc López / Anabel Medina Garrigues (withdrew)
See also: 15 and 1. 
Andy Ram / Elena Vesnina (second round)
See also: 16 and 3. Marcin Matkowski / 
Tathiana Garbin (second round)