The 2010 UniCredit Czech Open was a professional tennis tournament played on outdoor red clay courts. It was part of the 2010 ATP Challenger Tour. It took place in Prostějov, Czech Republic between 31 May and 5 June 2010.
| Nationality | Player | Ranking* | Seeding | |
|---|---|---|---|---|
| Radek Štěpánek | 20 | 1 | ||
| Albert Montañés | 34 | 2 | ||
| Victor Hănescu | 37 | 3 | ||
| Olivier Rochus | 62 | 4 | ||
| Jarkko Nieminen | 69 | 5 | ||
| Jan Hájek | 75 | 6 | ||
| Andrey Golubev | 77 | 7 | ||
| Michael Russell | 82 | 8 |
The following players received wildcards into the singles main draw:
The following players received entry from the qualifying draw:
See main article: 2010 UniCredit Czech Open – Singles. Jan Hájek def. Radek Štěpánek, 6–0, ret.
See main article: 2010 UniCredit Czech Open – Doubles. Marcel Granollers / David Marrero def. Johan Brunström / Jean-Julien Rojer, 3–6, 6–4, [10–6]