| Champ: |  Daria Gavrilova |
| Runner: | Yulia Putintseva |
| Score: | 6–3, 6–2 |
See main article: 2010 US Open (tennis).
The girls' singles tournament of the 2010 US Open started on Sunday September 5, the seventh day of the main tournament.
Heather Watson was the defending champion, but was no longer eligible to compete as a junior that year.
Daria Gavrilova won this event, after beating her compatriot Yulia Putintseva in the final, 6–3, 6–2.