The 2010 Tennis Napoli Cup was a professional tennis tournament played on outdoor red clay courts. It was part of the 2010 ATP Challenger Tour. It took place in Naples, Italy between 29 March and 4 April 2010.
| Nationality | Player | Ranking* | Seeding- | |
|---|---|---|---|---|
 Italy | Potito Starace | 73 | 1 | |
| Italy | Paolo Lorenzi | 90 | 2 | |
 Kazakhstan | Andrey Golubev | 93 | 3 | |
| Blaž Kavčič | 96 | 4 | ||
 Russian Federation | Igor Kunitsyn | 100 | 5 | |
 Spain | Pere Riba | 117 | 6 | |
 Romania | Victor Crivoi | 118 | 7 | |
| Rui Machado | 119 | 8 |
The following players received wildcards into the singles main draw:
The following players received entry from the qualifying draw:
The following players received the lucky spots:
See main article: 2010 Tennis Napoli Cup – Singles. Rui Machado def. 
Federico del Bonis, 6–4, 6–4
See main article: 2010 Tennis Napoli Cup – Doubles. 
Dustin Brown / 
Jesse Witten def. 
Rohan Bopanna / 
Aisam-ul-Haq Qureshi, 7–6(4), 7–5
