| Champ: |  Brian Dabul |
| Runner: |  Robby Ginepri |
| Score: | 4 - 6, 4 - 0, Ret. |
| Type: | singles doubles |
See main article: 2010 Tallahassee Tennis Challenger. John Isner was the defending of champion, but he chose not to participate this year.
Brian Dabul won in the final, after Robby Ginepri retired at the score of 6 - 4, 0 - 4.