The 2010 Tallahassee Tennis Challenger was a professional tennis tournament played on outdoor hard courts. It was part of the 2010 ATP Challenger Tour. It took place in Tallahassee, United States between April 17 and April 24, 2010.
| Nationality | Player | Ranking* | Seeding | |
|---|---|---|---|---|
| Rajeev Ram | 92 | 1 | ||
| Kevin Anderson | 103 | 2 | ||
| Taylor Dent | 106 | 3 | ||
| Robby Ginepri | 107 | 4 | ||
| Jesse Levine | 108 | 5 | ||
 Australia | Carsten Ball | 120 | 6 | |
| Kevin Kim | 133 | 7 | ||
| Ryan Sweeting | 142 | 8 |
The following players received wildcards into the singles main draw:
Jean-Yves Aubone Brendan Evans Bobby Reynolds Tim SmyczekThe following players received Special Exempt into the singles main draw:
The following players received entry from the qualifying draw:
Jamie Baker Joshua Goodall Dayne KellySee main article: 2010 Tallahassee Tennis Challenger – Singles. 
Brian Dabul def. Robby Ginepri, 4 - 6, 4 - 0, Ret.
See main article: 2010 Tallahassee Tennis Challenger – Doubles. Stephen Huss / Joseph Sirianni def. Robert Kendrick / Bobby Reynolds, 6 - 2, 6 - 4
