The 2010 Status Athens Open was a professional tennis tournament played on outdoor hard courts. It was part of the 2010 ATP Challenger Tour. It took place in Athens, Greece between 19 and 25 April 2010.
| Nationality | Player | Ranking* | Seeding | |
|---|---|---|---|---|
| Benjamin Becker | 40 | 1 | ||
| Philipp Petzschner | 49 | 2 | ||
| Marco Chiudinelli | 59 | 3 | ||
 Slovakia | Lukáš Lacko | 65 | 4 | |
 Israel | Dudi Sela | 70 | 5 | |
 Belgium | Xavier Malisse | 81 | 6 | |
| Rainer Schüttler | 91 | 7 | ||
| Lu Yen-hsun | 99 | 8 |
The following players received wildcards into the singles main draw:
The following players received entry from the qualifying draw:
The following players received the lucky loser spot:
See main article: 2010 Status Athens Open – Singles. Lu Yen-hsun def. Rainer Schüttler, 3–6, 7–6(4), 6–4
See main article: 2010 Status Athens Open – Doubles. Rik de Voest / Lu Yen-hsun def. Robin Haase / Igor Sijsling, 6–3, 6–4
