The 2010 Sparta Prague Open was a professional tennis tournament played on clay courts. It was part of the 2010 ITF Women's Circuit. It took place at TK Sparta Prague in Prague, Czech Republic between 10 and 16 May 2010 with a $50,000 prize money.
| Country | Player | Rank1 | Seed | |
|---|---|---|---|---|
 Czech Republic | Lucie Hradecká | 77 | 1 | |
| Czech Republic | Sandra Záhlavová | 86 | 2 | |
 Australia | Jelena Dokić | 106 | 3 | |
| Maša Zec Peškirič | 112 | 4 | ||
 Russian Federation | Ksenia Pervak | 118 | 5 | |
 Belarus | Darya Kustova | 135 | 6 | |
 Italy | Anna Floris | 151 | 7 | |
| Czech Republic | Andrea Hlaváčková | 154 | 8 |
The following players received wildcards into the singles main draw:
Petra Cetkovská Tereza Jankovská Aleksandra Romanova Kateřina KramperováThe following players received entry from the qualifying draw:
The following players received entry by a lucky loser spot:
See main article: 2010 Sparta Prague Open – Singles. Lucie Hradecká defeated Ajla Tomljanović, 6–1, 7–6(7–4)
See main article: 2010 Sparta Prague Open – Doubles. Ksenia Lykina / Maša Zec Peškirič defeated Petra Cetkovská / Eva Hrdinová, 6–3, 6–4
