The 2010 Serbia Open (also known as Serbia Open powered by Telekom Srbija for sponsorship reasons) was a men's tennis tournament played on outdoor clay courts. It was the 2nd edition of the event. It was part of the ATP World Tour 250 series of the 2010 ATP World Tour. It took place at the Milan Gale Muškatirović complex in Belgrade, Serbia, from 3 May through 9 May 2010.
The singles draw featured the tournament's host, ATP ranked No. 2 and defending champion Novak Djokovic. Other featured stars were 2010 Grand Prix Hassan II champion Stan Wawrinka, 2010 Regions Morgan Keegan Championships champion Sam Querrey and 2010 Heineken Open champion John Isner. Third-seeded Isner won the singles title.
| Player | Nationality | Ranking* | Seeding | |
|---|---|---|---|---|
| Novak Djokovic |  Serbia | 2 | 1 | |
| John Isner | 22 | 2 | ||
| Sam Querrey | 23 | 3 | ||
| Stanislas Wawrinka | 26 | 4 | ||
| Ivo Karlović | 30 | 5 | ||
| Viktor Troicki | Serbia | 37 | 6 | |
| Janko Tipsarević | Serbia | 38 | 7 | |
| Andreas Seppi | 47 | 8 |
The following players received wildcards into the main draw:
The following players received entry from the qualifying draw:
See main article: 2010 Serbia Open – Singles. 
Sam Querrey defeated John Isner, 3–6, 7–6(7–4), 6-4
See main article: 2010 Serbia Open – Doubles. 
Santiago González / Travis Rettenmaier defeated 
Tomasz Bednarek / Mateusz Kowalczyk, 7–6(8–6), 6–1
