| Champ: |  Andrej Martin |
| Runner: | Marek Semjan |
| Score: | 6–4, 7–5 |
| Type: | singles doubles |
See main article: 2010 Samarkand Challenger. Dustin Brown was the defending champion, but he chose to compete in Istanbul challenger instead.
Andrej Martin won the title, after won 6–4, 7–5 against his compatriot Marek Semjan in the final.
Óscar Hernández (first round)
Andrey Kuznetsov (second round)
Guillaume Rufin (first round) Evgeny Kirillov (second round) Andrej Martin (champion) Marek Semjan (final)