| Date: | August 9 – August 14 |
| Edition: | 14th |
| Location: | Samarkand, Uzbekistan |
| Champs: |  Andrej Martin |
| Champd: | Andis Juška / Deniss Pavlovs |
The 2010 Samarkand Challenger was a professional tennis tournament played on outdoor red clay courts. It was the fourteenth edition of the tournament which was part of the 2010 ATP Challenger Tour. It took place in Samarkand, Uzbekistan between 9 and 14 August 2010.
| Nationality | Player | Ranking* | Seeding | |
|---|---|---|---|---|
| Blaž Kavčič | 88 | 1 | ||
 Spain | Óscar Hernández | 158 | 2 | |
 Russian Federation | Andrey Kuznetsov | 167 | 3 | |
 France | Guillaume Rufin | 174 | 4 | |
| Russian Federation | Evgeny Kirillov | 205 | 5 | |
| Slovakia | Andrej Martin | 288 | 6 | |
| Slovakia | Marek Semjan | 297 | 7 | |
| Andis Juška | 312 | 8 |
The following players received wildcards into the singles main draw:
Tiago Fernandes
Murad Inoyatov Abduvoris Saidmukhamedov Vaja UzakovThe following players received a Special Exempt into the main draw:
The following players received entry from the qualifying draw:
See main article: 2010 Samarkand Challenger – Singles. Andrej Martin def. Marek Semjan, 6–4, 7–5
See main article: 2010 Samarkand Challenger – Doubles. Andis Juška / Deniss Pavlovs def. Lee Hsin-han / Yang Tsung-hua, 7–5, 6–3
