The 2010 Roma Open was a professional tennis tournament played on outdoor red clay courts. It was part of the 2010 ATP Challenger Tour. It took place in Rome, Italy between 19 and 24 April 2010.
| Nationality | Player | Ranking* | Seeding | |
|---|---|---|---|---|
| Florian Mayer | 45 | 1 | ||
| Daniel Brands | 86 | 2 | ||
 Austria | Daniel Köllerer | 93 | 3 | |
 Italy | Paolo Lorenzi | 101 | 4 | |
| Rui Machado | 119 | 5 | ||
| Frederico Gil | 121 | 6 | ||
 Spain | Rubén Ramírez Hidalgo | 125 | 7 | |
 Jamaica | Dustin Brown | 126 | 8 |
The following players received wildcards into the singles main draw:
The following players received special exempt into the singles main draw:
The following players received entry from the qualifying draw:
The following players received the lucky loser spot:
See main article: 2010 Roma Open – Singles. 
Federico del Bonis def. Florian Mayer, 6–4, 6–3
See main article: 2010 Roma Open – Doubles. Mario Ančić / Ivan Dodig def. Juan Pablo Brzezicki / Rubén Ramírez Hidalgo, 4–6, 7–6(8), [10–4]
