The 2010 Aberto de São Paulo was a professional tennis tournament played on outdoor hard courts. It was part of the 2010 ATP Challenger Tour. It took place in São Paulo, Brazil between 3 and 10 January 2010.
| Country | Player | Rank1 | Seed | |
|---|---|---|---|---|
 Brazil | Marcos Daniel | 87 | 1 | |
| Nicolás Massú | 112 | 2 | ||
 Argentina | Eduardo Schwank | 118 | 3 | |
| Brazil | Thiago Alves | 149 | 4 | |
| Argentina | Sebastián Decoud | 150 | 5 | |
| Brazil | Ricardo Mello | 151 | 6 | |
 Colombia | Carlos Salamanca | 158 | 7 | |
| Brazil | Júlio Silva | 165 | 8 |
The following players received wildcards into the singles main draw:
The following players received entry from the qualifying draw:
See main article: 2010 Prime Cup Aberto de São Paulo – Singles.
Ricardo Mello def. Eduardo Schwank, 6–3, 6–1See main article: 2010 Prime Cup Aberto de São Paulo – Doubles.
Brian Dabul / Sebastián Prieto def. 
Tomasz Bednarek / Mateusz Kowalczyk, 6–3, 6–3
