The 2010 UNICEF Open was a tennis tournament played on outdoor grass courts. It was the 21st edition of the UNICEF Open, and was part of the 250 Series of the 2010 ATP World Tour, and of the WTA International tournaments of the 2010 WTA Tour. Both the men's and the women's events took place at the Autotron park in Rosmalen, 's-Hertogenbosch, Netherlands, from 13 June through 19 June 2010. Sergiy Stakhovsky and Justine Henin won the singles titles.
See main article: 2010 UNICEF Open – Men's singles. 
Sergiy Stakhovsky defeated 
Janko Tipsarević 6–3, 6–0
See main article: 2010 UNICEF Open – Women's singles. 
Justine Henin defeated Andrea Petkovic, 3–6, 6–3, 6–4
See main article: 2010 UNICEF Open – Men's doubles. 
Robert Lindstedt / 
Horia Tecău defeated 
Lukáš Dlouhý / 
Leander Paes 1–6, 7–5, [10–7]
See main article: 2010 UNICEF Open – Women's doubles. 
Alla Kudryavtseva / 
Anastasia Rodionova defeated 
Vania King / 
Yaroslava Shvedova 3–6, 6–3, [10–6]
| Player | Nationality | Ranking* | Seeding | |
|---|---|---|---|---|
| Ivan Ljubičić | Croatia | 15 | 1 | |
| Marcos Baghdatis |  Cyprus | 26 | 2 | |
| Tommy Robredo |  Spain | 36 | 3 | |
| Viktor Troicki | Serbia | 39 | 4 | |
| Philipp Petzschner | Germany | 41 | 5 | |
| Thiemo de Bakker | Netherlands | 44 | 6 | |
| Janko Tipsarević | Serbia | 50 | 7 | |
| Benjamin Becker | Germany | 52 | 8 |
The following players received wildcards into the main draw:
The following players received entry from the qualifying draw:
The following player received the lucky loser spot:
| Player | Nationality | Ranking* | Seeding | |
|---|---|---|---|---|
| Justine Henin | Belgium | 18 | 1 | |
| Dinara Safina | Russia | 20 | 2 | |
| Maria Kirilenko | Russia | 28 | 3 | |
| Yaroslava Shvedova | Kazakhstan | 32 | 4 | |
| Alexandra Dulgheru | Romania | 31 | 5 | |
| Sara Errani |  Italy | 33 | 6 | |
| Andrea Petkovic | Germany | 34 | 7 | |
| Anabel Medina Garrigues | Spain | 38 | 8 |
The following players received wildcards into the main draw:
The following players received entry from the qualifying draw:
