The 2010 Košice Open was a professional tennis tournament played on outdoor red clay courts. It was part of the 2010 ATP Challenger Tour. It took place in Košice, Slovakia between 7 and 13 June 2010.
| Nationality | Player | Ranking* | Seeding | |
|---|---|---|---|---|
 Czech Republic | Jan Hájek | 75 | 1 | |
 Italy | Fabio Fognini | 92 | 2 | |
 Spain | Rubén Ramírez Hidalgo | 120 | 3 | |
| Spain | Óscar Hernández | 121 | 4 | |
 Kazakhstan | Mikhail Kukushkin | 135 | 5 | |
 France | David Guez | 136 | 6 | |
 Romania | Victor Crivoi | 158 | 7 | |
| Dieter Kindlmann | 162 | 8 |
The following players received wildcards into the singles main draw:
Mateusz Kowalczyk
Andrej Martin Miloslav Mečíř Jr. Martin PřikrylThe following players received entry from the qualifying draw:
Alexander Lobkov Jan Minář Marek Semjan
Artem SmirnovSee main article: 2010 Košice Open – Singles. Rubén Ramírez Hidalgo def. 
Filip Krajinović 6–3, 6–2
See main article: 2010 Košice Open – Doubles. Miloslav Mečíř Jr. / Marek Semjan def. 
Ricardo Hocevar / Caio Zampieri, 3–6, 6–1, [13–11]