| Date: | August 16 – August 21 |
| Edition: | 4th |
| Location: | Qarshi, Uzbekistan |
| Champs: |  Blaž Kavčič |
| Champd: |  Gong Maoxin / Li Zhe |
The 2010 Karshi Challenger was a professional tennis tournament played on outdoor hard courts. It was the fourth edition of the tournament which is part of the 2010 ATP Challenger Tour. It took place in Qarshi, Uzbekistan between 16 and 21 August 2010.
| Nationality | Player | Ranking* | Seeding | |
|---|---|---|---|---|
| Blaž Kavčič | 155 | 1 | ||
 Russian Federation | Konstantin Kravchuk | 156 | 2 | |
 Slovakia | Andrej Martin | 287 | 3 | |
| Slovakia | Marek Semjan | 296 | 4 | |
| Russian Federation | Andrey Kumantsov | 299 | 5 | |
| Andis Juška | 313 | 6 | ||
 Czech Republic | Jan Minář | 316 | 7 | |
 Spain | Carles Poch-Gradin | 323 | 8 |
The following players received wildcards into the singles main draw:
Murad Inoyatov Temur Ismailov Abduvoris Saidmukhamedov Vaja UzakovThe following players received entry from the qualifying draw:
See main article: 2010 Karshi Challenger – Singles. Blaž Kavčič def. 
Michael Venus, 7–6(6), 7–6(5)
See main article: 2010 Karshi Challenger – Doubles. Gong Maoxin / Li Zhe def. 
Divij Sharan / Vishnu Vardhan, 6–3, 6–1
