| Champ: |  Bob Bryan Mike Bryan |
| Runner: | John Isner Sam Querrey |
| Score: | 6–2, 6–3 |
See main article: 2010 Italian Open (tennis). Daniel Nestor and Nenad Zimonjić were the defending champions, but lost in the second round to Pablo Cuevas and Juan Mónaco.
Bob Bryan and Mike Bryan defeated their compatriots John Isner and Sam Querrey in the final. They won 6–2, 6–3.
All seeds receive a bye into the second round.