The women's 4 × 400 metres relay at the 2010 IAAF World Indoor Championships was held at the Aspire Dome on 14 March.
The Jamaican team came third and was awarded the bronze medals, but was later disqualified after Bobby-Gaye Wilkins was found to have been doping. A sample of hers collected at the championships was found positive for andarine.
| width=200 bgcolor=gold | Gold | width=200 bgcolor=silver | Silver | width=200 bgcolor=CC9966 | Bronze |
| Debbie Dunn DeeDee Trotter Natasha Hastings Allyson Felix | Denisa Rosolová Jitka Bartoničková Zuzana Bergrová Zuzana Hejnová | Kim Wall Vicki Barr Perri Shakes-Drayton Lee McConnell |
| Standing records prior to the 2010 IAAF World Indoor Championships | |||||
|---|---|---|---|---|---|
| World record | 3:23.37 | Glasgow, Great Britain | 28 January 2006 | ||
| Championship record | 3:23.88 | Budapest, Hungary | 7 March 2004 | ||
| World Leading | University of Arkansas | 3:33.18 | New York City, United States | 6 February 2010 | |
| Asian record | 3:37.46 | Doha, Qatar | 16 February 2008 | ||
| European record | 3:23.37 | Glasgow, Great Britain | 28 January 2006 | ||
| North and Central American and Caribbean record | 3:27.59 | Maebashi, Japan | 7 March 1999 | ||
| Oceanian Record | 3:26.87 | Maebashi, Japan | 7 March 1999 | ||
| Rank | Nation | Athletes | Time | Notes | |
|---|---|---|---|---|---|
| 3:27.34 | WL | ||||
| 3:30.05 | SB | ||||
| 3:30.29 | SB | ||||
| Doping | |||||
| Doping |