| Date: | July 19 – July 24 |
| Edition: | 16th |
| Location: | Lexington, Kentucky, United States |
| Champs: |  Carsten Ball |
| Champd: | Raven Klaasen / Izak van der Merwe |
The 2010 Fifth Third Bank Tennis Championships was a professional men's tennis tournament played on hard court. It was a sixteenth edition of the tournament which was part of the 2010 ATP Challenger Tour. It took place in Lexington, Kentucky, United States, between 19 July and 24 July 2010.
| Nationality | Player | Ranking* | Seeding | |
|---|---|---|---|---|
| Australia | Carsten Ball | 129 | 1 | |
 Lithuania | Ričardas Berankis | 132 | 2 | |
 Brazil | João Souza | 139 | 3 | |
| Kevin Kim | 152 | 4 | ||
| Jesse Levine | 158 | 5 | ||
| Paul Capdeville | 191 | 6 | ||
 Canada | Peter Polansky | 195 | 7 | |
| Australia | Greg Jones | 198 | 8 |
The following players received wildcards into the singles main draw:
Sekou Bangoura Jarmere Jenkins Denis Kudla Eric QuigleyThe following players received entry from the qualifying draw:
See main article: 2010 Fifth Third Bank Tennis Championships – Singles. Carsten Ball defeated Jesse Levine, 6–4, 7–6(7–1)
See main article: 2010 Fifth Third Bank Tennis Championships – Doubles. Raven Klaasen / Izak van der Merwe defeated Kaden Hensel / Adam Hubble, 5–7, 6–4, [10–7]
