The 2010 Estoril Open was a tennis tournament played on outdoor clay courts. It was the 21st edition of the Estoril Open for the men (the 14th for the women), and was part of the ATP World Tour 250 series of the 2010 ATP World Tour, and of the International-level tournaments of the 2010 WTA Tour. Both the men's and the women's events took place at the Estádio Nacional in Oeiras, Portugal, from 3 May until 9 May 2010. Albert Montañés and Anastasija Sevastova won the singles title.
| Player | Nationality | Ranking* | Seeding | |
|---|---|---|---|---|
| Roger Federer | Switzerland | 1 | 1 | |
 France | ||||
| Albert Montañés |  Spain | 31 | 4 | |
| Guillermo García López | Spain | 42 | 5 | |
| Florian Mayer | Germany | 48 | 6 | |
| Juan Ignacio Chela |  Argentina | 55 | 7 | |
| Pablo Cuevas | Uruguay | 56 | 8 |
The following players received wildcards into the main draw:
The following players received entry from the qualifying draw:
The following players received the lucky loser spot:
The following notable players withdrew from the event:
Ivan Ljubičić (left side strain) Gaël Monfils (stomach)| Player | Nationality | Ranking* | Seeding | |
|---|---|---|---|---|
| Ágnes Szávay |  Hungary | 33 | 1 | |
| Sorana Cîrstea |  Romania | 39 | 2 | |
| Aleksandra Wozniak |  Canada | 46 | 3 | |
| Anabel Medina Garrigues | Spain | 48 | 4 | |
| Melinda Czink | Hungary | 51 | 5 | |
| Magdaléna Rybáriková |  Slovakia | 53 | 6 | |
| Peng Shuai |  China | 54 | 7 | |
| Sybille Bammer |  Austria | 56 | 8 |
The following players received wildcards into the main draw:
The following players received entry from the qualifying draw:
The following player received the lucky loser spot:
See main article: 2010 Estoril Open – Men's singles. Albert Montañés defeated Frederico Gil, 6–2, 6–7(4–7), 7–5
See main article: 2010 Estoril Open – Women's singles. Anastasija Sevastova defeated Arantxa Parra Santonja, 6–2, 7–5
See main article: 2010 Estoril Open – Men's doubles. Marc López / David Marrero defeated Pablo Cuevas / Marcel Granollers, 6–7(1–7), 6–4, [10–4]
See main article: 2010 Estoril Open – Women's doubles. Sorana Cîrstea / Anabel Medina Garrigues defeated 
Vitalia Diatchenko / Aurélie Védy, 6–1, 7–5
